TAY Wei Hong

TAY Wei Hong

B.Sc., (Hons)
Nanyang Technological University
whtay011 [at] e.ntu.edu.sg

Wei Hong completed his bachelor’s degree in the School of Biological Sciences at Nanyang Technology University. Prior to this, he received his Diploma (Merit) in the Applied Science School at Temasek Polytechnic. He previously worked at the laboratory of Toshiro Ito in Temasek Life Sciences Laboratory, where he investigated the role of lipids in plant flowering. He later joined the Human Cognition and Human Factors group at the Defense Medical and Environmental Research Institute as an assistant research officer, under the supervision of Dr. Fredrick Tey. He is passionate about optical and electron microscopy, and the use of correlative light and electron techniques. He is a member of the Royal Microscopical Society (RMS) and the Microscopy Society of Singapore (MSS). When not in the lab, Wei Hong enjoys reading novels, the theater, and attending piano concerts. He enjoys baking cakes for the lab and enjoys a good cup of tea.

Extensive clinical and epidemiological studies have identified Enterococcus faecalis as one of the most frequently isolated bacteria species in wounds. Unfortunately, its presence in wounds is largely ignored in the clinic and remains poorly understood. Considering that healthcare associated infections affect close to 1.7 million people in the US yearly, and that 25% of these are attributed to wound infections, there is a pressing need to understand the pathogenesis of E. faecalis infections with regards to wounds.

Wei Hong’s work focuses on using an in-vitro tissue culture model system to understand the pathogenic strategies of E. faecalis. Currently, he is investigating Enterococcal persistence within mammalian cells. He is interested in the intracellular life of E. faecalis. The study employs the use of correlative light and electron microscopy techniques, in addition to siRNA knockdowns, lentiviral transduction, cytokine/chemokine profiling and RNA-Seq.

Wei Hong currently manages the tissue culture facility in SCELSE together with the management team. He is also involved in the establishment and management of procurement contracts for the lab. Wei Hong is also currently working on the following projects:

Collaboration with Kelvin Chong:

  1. Characterization of Enterococcal wound infections using a mouse model of wound infection. Wei Hong has applied both scanning electron and confocal microscopy to determine the spatial localization of E. faecalis in infected wounds.

Collaboration with Brenda Tien:

  1. Mechanism of immune modulation by E. faecalis in macrophages. Wei Hong applied confocal and SIM to study mechanisms of immune modulation ion infected macrophages.

Collaboration with Anuradha Vajjala:

  1. Development of in vitro and in vivo models to characterize biofilm formation by a necrotizing strain of group A Streptococcus. Wei Hong performed scanning electron microscopy to study biofilm formation on infected mammalian cells.

1. Chong KKL*, Tay WH*, Janela B, Yong MHA, Liew TH, Madden L, Keogh D, Barkham T, Ginhoux F, Becker DL, Kline KA. Enterococcus faecalis Modulates Immune Activation And Slows Healing During Wound Infection. bioRxiv. 2017 Apr 7. doi: https://doi.org/10.1101/125252. (* equal contribution)
2. Keogh D, Tay WH, Ho YY, Dale JL, Chen S, Umashankar S, Williams RB, Chen SL, Dunny GM, Kline KA. Enterococcal Metabolite Cues Facilitate Interspecies Niche Modulation and Polymicrobial Infection. Cell Host Microbe. 2016 Oct 12;20(4):493-503. doi: 10.1016/j.chom.2016.09.004.
3. Tay WH, Chong KK, Kline KA. Polymicrobial-Host Interactions during Infection. J Mol Biol. 2016 Aug 28;428(17):3355-71. doi: 10.1016/j.jmb.2016.05.006. Epub 2016 May