Overview
Our laboratory is working to understand Enterococcus faecalis and Enterococcus faecium factors that contribute to colonization, biofilm formation, and infections such as wound and urinary tract infection, intestinal colonization and expansion, and infective endocarditis. Our current research projects span four main areas:
Regulation and Biogenesis of the Enterococcal Cell Envelope
Using genetic, biochemical, and structural approaches, we investigate the regulatory networks and molecular mechanisms that govern the assembly and remodeling of the Enterococcal cell envelope, including its lipid composition, surface structures, and protein secretion systems.
AMR and Polymicrobial Biofilm-Associated Infection
Using in vitro and in vivo infection models, genetic screens, and transcriptomic profiling, we investigate how E. faecalis forms biofilms alone or with other pathogens, and how these communities exhibit altered antimicrobial tolerance and virulence during infection.
Cell Biology of Enterococcal Infection
Leveraging cell- and tissue-based models, high-resolution and live-cell imaging, and genetic tools, we explore the intracellular life cycle of E. faecalis, focusing on mechanisms of host cell entry, survival, replication, and manipulation of innate immune responses.
Therapeutic Targeting of Enterococcal Infection
Using preclinical models, we identify and evaluate novel therapeutic strategies to combat Enterococcal infection, including immunomodulatory approaches that enhance host defense, antivirulence strategies, and precision antimicrobials such as bacteriophages.
