🇸🇬 CHOO Pei Yi

 

CHOO Pei Yi

M.Sc., Nanyang Technological University
PYChoo [at] ntu.edu.sg




Pei Yi is optimist and brings her camera along wherever she goes because she likes to capture memories of the places she goes and the people she meets. Pei Yi is an animal lover, which also explains why she became a vegetarian. During her free time, she likes to play with her dog, watch movies, and listen to music. In the lab, Pei Yi is working to characterize factors that regulate virulence factor assembly in E. faecalis. Pei Yi is also one of the lab’s imaging gurus.


My project involves looking at the spatial and temporal organization of sortase assembled pili in E. faecalis. The main techniques involved in this study are immunofluorescence imaging using widefield and super resolution microscopes to examine the localization of the pili on the surface of the cell and on the cell membrane under different conditions. This study allows us to further understand and characterize the sorting of virulence factors to the E. faecalis cell wall.

1. The HtrA chaperone monitors sortase-assembled pilus biogenesis in Enterococcus faecalis. Colomer-Winter C, Yong AMH, Chong KKL, Veleba M, Choo PY, Gao IH, Matysik A, Ho FK, Chen SL, Kline KA. (2024) PLoS Genetics, 20(8), e1011071. doi:10.1371/journal.pgen.1011071
2. An essential protease, FtsH, influences daptomycin resistance acquisition in Enterococcus faecalis. Nair ZJ, Gao IH, Firras A, Chong KKL, Hill E D, Choo PY, Colomer-Winter C, Chen Q, Manzano C, Pethe K, Kline KA. (2024) Molecular microbiology,10.1111/mmi.15253.
3. Mitoxantrone targets both host and bacteria to overcome vancomycin resistance in Entercoccus faecalis. da Silva RAG, Wong JJ, Antypas H, Choo PY, Goh K, Jolly S, Liang C, Tay Kwan Sing L, Veleba M, Hu G, Chen J, Kline KA. (2023) Science Advances. Vol. 9, Issue. 8.
4. Spatial and temporal surface deposition of sortase-assembled pili in Enterococcus faecalis. Choo PY, Yang CW, VanNieuwenhze MS, Kline KA. (2023) Molecular Microbiology Vol. 118, No. 5
5. Escherichia coli BarA-UvrY regulates the pks island and kills Staphylococci via the genotoxin colibactin during interspecies competition. Wong JJ, Ho FK, Choo PY, Chong KLC, Ho BCM, Neelakandan R, Keogh D, Barkham T, Chen J, Liu CF, Kline KA. (2022) PLoS Pathog. Sep 6;18(9):e1010766.
6. Enterococcus faecalis alters endo-lysosomal trafficking to replicate and persist within mammalian cells. da Silva RAG, Tay WH, Ho FK, Tanoto FR, Chong KKL, Choo PY,
Ludwig A, Kline KA. (2022). PLoS Pathog 18, e1010434
7. Enterococcus faecalis adapts to antimicrobial conjugated oligoelectrolytes by lipid rearrangement and differential expression of membrane stress response genes. Chilambi GS, Hinks J, Matysik A, Zhu X, Choo PY, Liu X, Chan-Park MB, Bazan GC, Kline KA, Rice SA. (2020) Front Microbiol